Influence of Dietary Inositol Supplementation on Growth, Liver Histology, Lipid Metabolism, and Related Genes Expression on Juvenile Hybrid Grouper (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatu) Fed High-Lipid Diets

An 8-week feeding trial was conducted to investigate the effects of dietary inositol on growth performance, lipid metabolism, and expression related genes hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatu) (initial body weight 6.76 ± 0.34 g). Six isonitrogenous isolipidic (16.35%) diets were formulated contain graded levels (0.17 g/kg (J1), 0.62 (J2), 1.03 (J3), 1.78 (J4), 3.43 (J5), 6.59 (J6)). The results indicated that (a) gain rate appeared gradually increasing trend significant increase in four treatments (J3, J4, J5, J6) ( id="M2"> P < 0.05 ). Protein efficiency ratio slightly improved J4 group id="M3"> ), but there no difference exhibiting survival between inositol-treated groups control id="M4"> > (b) In serum, significantly reduced contents triglyceride (TG), total cholesterol (TC), low-density lipoprotein, clearly increased content high-density lipoprotein id="M5"> liver, decreased TG, TC, very id="M6"> activities lipase, hepatic adipose lipase id="M7"> (c) With supplement, decreasing droplet could be observed hepatocyte. (d) downregulated mRNA fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), 6-phosphogluconate dehydrogenase (6PGD), sterol regulatory element-binding protein-1 (SREBP1) id="M8"> while it remarkably upregulated (LPL), hormone-sensitive (HSL), carnitine palmitoyl transferase1 (CPT1) id="M9"> summary, composition liver through synthesis catabolism. Under experimental conditions, broken-line regression analysis showed 1.23~1.67 recommended juvenile high-lipid diets.